Thapsigargin and di-tert-butylhydroquinone induce synergistic stimulation of DNA synthesis with phorbol ester and bombesin in Swiss 3T3 cells.
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چکیده
منابع مشابه
Bombesin stimulation of DNA synthesis and cell division in cultures of Swiss 3T3 cells.
Bombesin is shown to be a potent mitogen for Swiss 3T3 cells. At nanomolar concentrations the peptide markedly enhances the ability of fresh serum to stimulate DNA synthesis in confluent and quiescent cultures of these cells. In the presence of a low concentration (3.5%) of serum, bombesin stimulates 3T3 cell proliferation. In serum-free medium, bombesin induces DNA synthesis in the absence of ...
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15 صفحه اولActivity of phorbol ester tumor promoters on enucleated Swiss 3T3 cells.
The site of action of the phorbol esters, potent tumor promoters, is not known. To evaluate the possible role of the nucleus in the specific interaction of phorbol esters with cells, the activity of phorbol-12-myristate-13-acetate was determined on intact and enucleated Swiss 3T3 mouse cells. The parameter which was used as a measure of cellular response was the morphological change of transien...
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When pBR322 is manually microinjected into the nuclei of quiescent Swiss 3T3 cells it stimulates the incorporation of [3H]thymidine into DNA. The evidence clearly shows that this increased incorporation that is detected by in situ autoradiography in microinjected cells represents cellular DNA synthesis and not DNA repair or plasmid replication. The effect is due to pBR322 and not due to impurit...
متن کاملA peptide that inhibits the mitogenic stimulation of Swiss 3T3 cells by bombesin or vasopressin.
The synthetic peptide [D-Arg1,D-Pro2,D-Trp7,9,Leu1]substance P inhibits the stimulation of DNA synthesis induced in Swiss 3T3 cells by bombesin or vasopressin, but not that induced by a wide range of other growth factors and mitogens. The stimulation induced by 10 pM-3 nM-bombesin is inhibited by 1-30 microM-antagonist in a manner consistent with competition at the bombesin receptor. The inhibi...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1994
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)31666-1